Mukherjee breathed last at around 9.35 AM on Sunday, family members said. He is survived by wife and two daughters.
He began his acting career in the play "Khudha" and his first film was Mrinal Sen's 1958 film "Neel Akasher Neechey". He had earned plaudits with his roles in Satyajit Ray's 'Joy Baba Felunath' and 'Ganashatru'.
Mukherjee was also trained as a Tabla player by the famous Tabla player Krishnakumar Ganguly.
West Bengal Chief Minister Mamata Banerjee expressed her grief on the demise of the veteran actor.
"Saddened at the passing away of veteran theatre and film actor Manu Mukherjee. We conferred on him the Lifetime Achievement Award at the Tele-Samman Awards 2015. My condolences to his family, colleagues and admirers," the Chief Minister tweeted.